Reporter

Part:BBa_K911004:Design

Designed by: Thomas Whittaker   Group: iGEM12_Cambridge   (2012-09-21)


Synthesised Ratiometric Luciferase construct in non-standard plasmid


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 3988
    Illegal NheI site found at 7654
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1479
    Illegal BglII site found at 4330
    Illegal BglII site found at 6912
    Illegal BglII site found at 8389
    Illegal BamHI site found at 7593
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 6308
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Sequence designed to be compatible with bacillus and e.coli. As such, all genes codon-optimised for bacillus, all RBSes are at bacillus consensus, all promoters are documented as having the same function in e.coli and bacillus.


Source

Commercial synthesis by DNA 2.0

References